Taxonomic identification report

Sample ID: KDD0161

Department of Agriculture, Fisheries and Forestry logo

[analysis description + link to github repo]

Facility Hogwarts
Analyst John Doe
Analysis started 2025-05-03 01:28:01
Analysis completed 2025-05-03 01:28:01
Wall time 0:0:0 hours

Result overview

Tetranychus marianae

Outcome: Positive species identification.

Reasoning: [Flag 1A] 1 candidate species matched with high stringency (identity ≥ 98.5%).

Preliminary morphology ID confirmed? True

Flag 7A: Identified species is consistent with preliminary morphology ID Tetranychus.

Taxa of interest detected? True

Flag 2A: Taxon of interest detected in candidate species

Flag 5.1A: The given locus for this taxon is well represented in reference database (>5 entries)

Flag 5.2B: 10-90% of related taxa have reference sequence(s) at the given locus

Sample details

Locus COI
Preliminary ID Tetranychus
Taxa of interest Tetranychus
Country Australia
Host Aibika
Sample ID KDD0161
Query DNA sequence

Analyst evaluation

Identification of candidate species

Flag 1A: Positive species identification - Tetranychus marianae
1 candidate species matched with high stringency (identity ≥ 98.5%)

Candidate hits must meet ONE of these criteria:

Minimum alignment length 400bp
Minimum query coverage 85.0%

Candidate hits have then been classified as follows:

Classification Alignment identity Number of hits Number of species
STRONG MATCH ≥ 98.5% 6 1
MODERATE MATCH ≥ 93.5% NA NA
NO MATCH < 93.5%

Candidate species

Species Hits Identity E-value Database coverage
Tetranychus marianae 6 98.9% 0.0

Analyst evaluation

Taxa of interest (TOI)

This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest Match rank Match taxon Match species Match accession Match identity Database coverage
Tetranychus genus Tetranychus Tetranychus marianae MW326470 0.989

Reference sequence source diversity

This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.

Analyst evaluation

Independent sources

Flag 4B: Reference sequence sources lack diversity and may therefore be unreliable
Reasoning: Matching sequence records for this species have only 1-5 independent sources (found 2 sources)

2 Independent Sources

The matching reference sequences for this species have been annotated by 2 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession Automated Authors Title Journal
MW326470 False Sharkey,E.R.
Bolton,S.J.
Moore,M.R.
McVay,J.D.
Beaulieu,F. 		Morphological and molecular data reveal the conspecificity of Tetranychus gloveri and Tetranychus okinawanus (Acari: Trombidiformes: Tetranychidae) Unpublished
MW326470 False Moore,M.R.
Roberts,C.G.
Combee,L.A.
Sharkey,E.R. 		Direct Submission Submitted (02-DEC-2020) Division of Plant Industry, Florida Department of Agriculture and Consumer Services, 1911 SW 34th St, Gainesville, FL 32608, USA
MW326471 False Sharkey,E.R.
Bolton,S.J.
Moore,M.R.
McVay,J.D.
Beaulieu,F. 		Morphological and molecular data reveal the conspecificity of Tetranychus gloveri and Tetranychus okinawanus (Acari: Trombidiformes: Tetranychidae) Unpublished
MW326471 False Moore,M.R.
Roberts,C.G.
Combee,L.A.
Sharkey,E.R. 		Direct Submission Submitted (02-DEC-2020) Division of Plant Industry, Florida Department of Agriculture and Consumer Services, 1911 SW 34th St, Gainesville, FL 32608, USA
MW326472 False Sharkey,E.R.
Bolton,S.J.
Moore,M.R.
McVay,J.D.
Beaulieu,F. 		Morphological and molecular data reveal the conspecificity of Tetranychus gloveri and Tetranychus okinawanus (Acari: Trombidiformes: Tetranychidae) Unpublished
MW326472 False Moore,M.R.
Roberts,C.G.
Combee,L.A.
Sharkey,E.R. 		Direct Submission Submitted (02-DEC-2020) Division of Plant Industry, Florida Department of Agriculture and Consumer Services, 1911 SW 34th St, Gainesville, FL 32608, USA
MW326473 False Sharkey,E.R.
Bolton,S.J.
Moore,M.R.
McVay,J.D.
Beaulieu,F. 		Morphological and molecular data reveal the conspecificity of Tetranychus gloveri and Tetranychus okinawanus (Acari: Trombidiformes: Tetranychidae) Unpublished
MW326473 False Moore,M.R.
Roberts,C.G.
Combee,L.A.
Sharkey,E.R. 		Direct Submission Submitted (02-DEC-2020) Division of Plant Industry, Florida Department of Agriculture and Consumer Services, 1911 SW 34th St, Gainesville, FL 32608, USA
MW672202 False Sharkey,E.R.
Moore,M.R.
Bolton,S.J.
Beaulieu,F. 		Morphological and molecular data reveal the conspecificity of Tetranychus gloveri and Tetranychus okinawanus (Acari: Trombidiformes: Tetranychidae) Unpublished
MW672202 False Moore,M.R.
Roberts,C.G.
Combee,L.A.
Sharkey,E.R. 		Direct Submission Submitted (26-FEB-2021) Division of Plant Industry, Florida Department of Agriculture and Consumer Services, 1911 SW 34th St, Gainesville, FL 32608, USA
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Source 2

Hit accession Automated Authors Title Journal
OP703558 False Bhaskar,H.
Mohan S,M.
Sajimon,B. 		Direct Submission Submitted (23-OCT-2022) Agricultural Entomology, Kerala Agricultural University, Vellanikkara, Thrissur, Kerala 680656, India

Intraspecies diversity

This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.

Analyst evaluation

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The phylogetic tree was constructed with FastME using the Neighbor-Joining method. Multiple-sequence alignment of the candidate reference sequences was performed using MAFFT. The visualization is rendered with TidyTree.

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.

Taxa Database
General GBIF
General ITIS
Mealybugs & scale ScaleNet database
Thrips Thripswiki
Spider Mites Spider Mites Database
Psocodea (Barklice, Booklice, and Parasitic Lice) Psocodea Species File Online
Orthoptera Orthoptera Species File Online
Drosophilidae TaxoDros
Diptera Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum
Aphids Aphid Species File
Ants AntWeb
AntCat
Lepidoptera (butterflies and moths) The Global Lepidoptera Names Index
Gracillariidae (primitive moths) Global Taxonomic Database of Gracillariidae
Pyralidae (pyralid moths) Global Information System on Pyraloidea
Tortricidae (tortrix moths) Tortricidae Resources on the Net